Concepts of validation and Good Manufacturing Practice (GMP) are introduced
* Helps students to better understand regulatory constraints on bioprocess development
Updated coverage of concepts
* Shows students the connection between traditional ideas and emerging areas—such as tissue engineering and gene therapy
Material on functional genomics and cellular engineering
* Provides students with new developments in biology as they impact bioprocess engineering
Expanded discussion of modeling approach
* Presents students with a clarified section on models in continuous cultures and adds cybernetic modeling
Expanded coverage of chromatography
* Introduces students to discussions of IMAC (immobilized metal affinity chromatography), use of fusion proteins, and porous supports
Expanded sections on metabolic engineering, animal cell culture, and protein processing
* Offers students information on analysis of metabolic pathways, bioreactor considerations for animal cells, and includes some recent examples
Additional examples and homework problems—e.g., on topics such as enzyme reaction; reactor operation and scale-up; purification; waste treatment; and genetically engineered cells
* Enables students to more thoroughly test their understanding of applied concepts
Reorganized coverage
* Gives students a more logical presentation of genetic instability, strategies for genetic engineering, and then an approach to selection of host expression system for production of a heterologous protein
Table of Contents I. INTRODUCTION
1. What is a Bioprocess Engineer
II. THE BASICS OF BIOLOGY: AN ENGINEER'S PERSPECTIVE
2. An Overview of Biological Basics
3. Enzymes
4. How Cells Work
5. Major Metabolic Pathways
6. How Cells Grow
7. Stoichiometry of Microbial Growth and Product Formation
8. How Cellular Information is Altered
III. ENGINEERING PRINCIPLES FOR BIOPROCESSES
9. Operating Considerations for Bioreactors for Suspension and Immobilized Cultures
10. Selection, Scale-Up, Operation, and Control of Bioreactors
11. Recovery and Purification of Products
IV. APPLICATIONS TO NONCONVENTIONAL BIOLOGICAL SYSTEMS
12. Bioprocess Considerations in Using Animal Cell Cultures
13. Bioprocess Considerations in Using Plant Cell Cultures
14. Utilizing Genetically Engineered Organisms
15. Medical Applications of Bioprocess Engineering
16. Mixed Cultures
17. Epilogue
DR. MICHAEL L. SHULER is Professor in the School of Chemical Engineering, Cornell University. His areas of research include structured models, heterologous protein expression systems, cell culture analogs for pharmacokinetic models, in-vitro toxicology, plant-cell tissue culture, microbial functional genomics, and bioremediation.